|
Gas solubilities in microbial culture media2016-09-12 16:34
微生物培养基中(威正翔禹)的气体溶解度的可用信息。强调的是对氧气和二氧化碳的溶解度。可以成功地应用于培养基的实验技术。所有的参数,影响气体的溶解度,即上述所有媒体的组成进行了深入的探讨。在一般情况下,在营养培养基可以通过对数相加的方法预测气体的溶解度。为此,知识的传播和溶解度参数(K1)组成的单个化合物是必需的。对于各种遇到的培养液参数K1氧物质可以从文献数据的评估和总结。提出适当的建议,应用直接和间接的预测方法。失败的案例也被提及。 英文原文: Available information on gas solubility in microbial culture media is reviewed. Emphasis is given to oxygen and carbon dioxide solubilities. Experimental techniques which can be successfully applied to culture media are presented. All the parameters which affect gas solubilities, i.e., above all the composition of the media are thoroughly discussed. In general, gas solubilities in nutrition and cultivation media can be predicted by a log-additivity approach. To this end knowledge of the composition of the media and the solubility parameters (K1) of the individual compounds is required. For a variety of substances encountered in cultivation broths the parameters K1 for oxygen could be evaluated from literature data and are summarized in this paper. Appropriate recommendations for applying direct and indirect predictive methods are given. Cases of failure are mentioned as well.
|