比较研究胎牛血清和马血清对初级马支气管成纤维细胞的生长和分化的影响

从消化支气管组织细胞产量是一致的。在这两种培养条件，EBF用台盼蓝染色和活细胞的百分率是相似的，通常>95％。下倒光镜分析，观察细胞坏死或凋亡的细胞没有显著的证据。对于细胞形态，EBF在DMEM中培养，用10％FBS的似乎是通常扁平和纺锤形（具有均匀的细胞质）在几个星期的通道相比EBF在DMEM中培养，用10％的HS（图1的A）。

在FBS的培养基，EBF生长在生长表面只有松散的细胞-细胞接触，直到达到汇合，然后形成了仍然是典型的成纤维细胞单层，直到通道15道之间EBF紧平行线16-20开始改变其形态：细胞大，平坦和更多边形的形状，具有大，异质核。同时，细胞生长迅速降低和细胞存活率降低。与此相反，EBF在HS的存在下培养的表现出内培养2天改变形态学变化;细胞小，形状在细胞质颗粒剂般黑暗的结构（图结合更紧凑1C）。

此外，细胞生长集群和链条，未能在一周内达到汇合。EBF的这种形态行为这种文化条件下看到，直到第7代，但此后，EBF未能定期增殖，并在自己的生存能力（通道9）下降。在这两种培养条件下，最EBF（>99％）为阳性波形蛋白，但与细胞质内的培养基含有FBS的（图更特性丝状结构1B）的比在HS（图的存在1D）.

总之，血清中除基础EBF培养基提高EBF分化成肌成纤维细胞，而这些发现开发出模拟体内的生理过程，并研究气道疾病的机制和重塑体外成纤维细胞培养模型有用。

英文原文：

Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts

Primary EBF were cultured on non-collagen coated flasks without serum or in the presence of fetal bovine serum (FBS) or horse serum (HS) or in serum depleted medium. EBF cultured in serum-free basal media and those serum deprived were not able to proliferate and even exhibited considerable cell death. In media containing FBS or HS, proliferation of the cells was reproducible between different primary cultures and cells demonstrated expression of vimentin. Large variations were found in the ability of FBS and HS to support growth and differentiation of EBF in monolayer culture. Indications of growth-promoting actions, increasing passage number as well as maintaining fibroblast morphology were found rather in FBS than in HS. EBF culturing in HS needed longer doubling and confluence time. The protein content of the cell pellets was higher in EBF cultured in medium containing HS than FBS. Alpha-smooth muscle actin seemed to be less expressed in EBF cultured in medium containing FBS than those in HS.