有效的抗支原体抗生素

为了根除FBS和胰蛋白酶的支原体，UV照射比所提及的方法更有效。与γ照射想比，UV照射不损害血清组分。紫外线照射可以消除胰蛋白酶或FBS任何潜在的污染。

需要经常检查以确认支原体?是否完全根除。有时支原体被抑制并且它们的滴度降低到可检测的水平以下。因此，采用敏感的支原体检测手段对于探知细胞培养物中的任何再感染是必要的。建议在至少四至六次传代后，重新检查在不含抗生素的培养基培养的细胞。（需要支原体祛除试剂，请点击这里！）

英文原文：

Prevention and Detection of Mycoplasma Contamination in Cell Culture

Various antibiotics with different inhibitory effects on cellular metabolism can be helpful to eliminate mycoplasma contamination. The mechanism of action of macrolides and tetracyclines is inhibiting protein synthesis, but they bind to different subunits of ribosomes. The quinolone inhibits the DNA replication by obstructing bacterial gyrase. In the case of using just one type of antibiotic, it is highly possible that mycoplasmas escape from the inhibitory mechanism or become resistant to it. Insufficient duration or concentration of antibiotic treatment can cause resistance. It is because of surviving the resistant mycoplasmas in the presence of low amount of antibiotics. These resistant mycoplasmas can neutralize the inhibitory mechanism of antibiotic or change its attack site. They can also pump the antibiotic out (18).

The efficiency of antibiotics in elimination of mycoplasmas is between 66 and 85 percent. These percentages include the cultures in which the growth of eukaryotic cells was inhibited, though. Three to 11 percent of cells which are already in a bad condition with a high infection level are lost after antibiotic treatment. However, this event depends on the antibiotic (65).

Elimination of mycoplasmas is usually difficult or unsuccessful due to the resistance of mycoplasmas to antibiotics. It is more successful to passage mycoplasma contaminated cells in nude mice; however, the recovery of cells is not always guaranteed. But when the cells can be collected from subcutaneous tumors in nude mice, the cells are free from mycoplasmas together with a large number of macrophages. Since there is no thymus in nude mice and surely no T-cell dependent immune response, it is possible the macrophages are in charge of the elimination of mycoplasmas. This hypothesis was proved by a brief co-cultivation of mycoplasma contaminated cells with mouse macrophages (75, 76).