细胞培养支原体污染：发生率，来源，效果，检测，消除，预防

Here we provide a concise overview of the current knowledge on:

(1) the incidence and sources of mycoplasma contamination in cell cultures, the mycoplasma species most commonly detected in cell cultures, and the effects of mycoplasmas on the function and activities of infected cell cultures;

(2) the various techniques available for the detection of mycoplasmas with particular emphasis on the most reliable detection methods;

(3) the various methods available for the elimination of mycoplasmas highlighting antibiotic treatment;

(4) the recommended procedures and working protocols for the detection, elimination and prevention of mycoplasma contamination. The availability of accurate, sensitive and reliable detection methods and the application of robust and successful elimination methods provide powerful means for overcoming the problem of mycoplasma contamination in cell cultures.

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