微米在细胞分离纳米

广泛的细胞分离技术本文中描述说明了高水平的兴趣和活动在这一领域。描述的大小和基于密度的方法提供了一个潜力巨大的分离细胞亚种群的特定的标记不清楚或无法使用(例如防止细胞激活)。

基于吸引力的方法(荧光、磁、结合力和电泳)可以使用快速(~分钟)和连续分离高特异性(~ 99%)。的所有方法，设备的设计是这样的，他们可以在大规模并行的方式增加规模和吞吐量的前提下纯度和效力。

此外，微流体分离系统可以很容易地整合与单细胞等设备，执行下游分析裂解和蛋白质组和基因组分析。先进水平的设计、制造和测量功能，我们预计，在未来几年重点将从“概念验证”的原型设备，很容易在经济生产和应用，如即时临床诊断方法、药物发现，化学生物学代理检测。

The broad spectrum of cell separation technologies described in this review illustrates the high level of interest and activity in this area. The described size- and density-based approaches offer a great potential for separation of cell subpopulations for which specific markers are not known or cannot be used (eg, to prevent cell activation). Affinity-based approaches (fluorescence-, magnetic-, adhesion-based, and electrophoretic) can be employed for fast (~minutes) and continuous separation with high specificity (~99%). For all of the approaches, the design of the devices is such that they can be operated in a massively parallel fashion to increase scale and throughput without compromising purity and efficacy.