微生物培养国际流行 — 非动物源性共增菌培养基：产品详细说明

产品1、植物源性TSB

（Soyabean HiVeg Medium ）

货号：MV011

适合大肠杆菌、沙门氏菌、李斯特菌、金黄色葡萄球菌、肺炎链球菌、白色念珠菌、巴西曲霉菌、铜绿假单胞菌、枯草芽孢杆菌等大多数菌的生长繁殖。

Soyabean HiVeg Medium is a general purpose medium used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.

大豆植物源性培养基是通用型培养基，用于广泛培养各种微生物。也推荐用于霉菌和细菌的无菌检查。

Composition**  （构成成分）

Directions （说明）

Suspend 30 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 25°C.

1000ml水中加30g培养基，如果需要可加热使其完全溶解，然后进行分装即可。高压灭菌 （15磅121℃） 15分钟。冷却至25℃。

Note: If any fibres are observed in the solution, it is recommended to filter the solution through a 0.22 micron filter to eliminate the possibility of presence of fibres.

注：若溶液中有纤维，建议用0.22μm的滤膜过滤。

Principle And Interpretation （原理和说明）

Soyabean HiVeg Medium is prepared by completely replacing animal based peptones with vegetable peptones that makes the medium free of BSE/TSE risks. It is the modification of Soyabean Casein Digest Medium recommended by various pharmacopeias for sterility testing of various products and sensitivity testing of antimicrobial agents by tube dilution method (1-3) . This is a very nutritious medium supporting the growth of a variety of organisms (4). The combination of HiVeg hydrolysate and papaic digest of soyabean meal makes this medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and dipotassium phosphate serves as the carbohydrate source and the buffer in the medium. Sodium chloride maintains the osmotic balance of the medium.

此产品是植物源性蛋白胨配置而成，可完全取代动物源性蛋白胨，从而避免疯牛病的风险。它是各种药典推荐进行无菌检查和药敏实验用的胰蛋白胨大豆肉汤培养基的改良型。它是一种非常富有营养的培养基，支持各种微生物的生长。植物源蛋白水解物和大豆粉水解物的结合， 可提供微生物生长所必需的氨基酸和长链多肽。葡萄糖和K₂HPO₄分别提供碳源及缓冲，Nacl维持渗透压平衡。

Quality Control（质量控制）

Appearance（外观）

Cream to yellow homogeneous free flowing powder

乳黄色均一自由流动的粉末。

Colour and Clarity of prepared medium

Light yellow coloured clear solution without any precipitate.

淡黄色澄清溶液，无沉淀。

Reaction

pH of 3.00% w/v aqueous solution at 25°C (after sterilization). pH : 7.3±0.2

在25℃且3%的水溶液条件下，灭菌后其PH值为7.3±0.2。

pH

7.10-7.50

Stability test 稳定性

Light yellow coloured clear solution without any precipitation or sedimentation at room temperature for 7 days

室温存放7天，仍呈现淡黄色、澄清、无沉淀的溶液。

Growth promoting properties （生长支持性）

Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu(at 30-35°C for 18-24 hours for bacteria and 5 days for fungal). Growth promotion is carried out as per USP/EP/BP/JP.

清晰可见的微生物的生长与先前测试和批准的培养基批号所获得的微生物相当。培养条件为接种≤100cfu，在30-35℃下细菌培养18-24小时，真菌培养5天。生长促进流程按《美国药典》、《欧洲药典》、《英国药典》、《日本药典》进行。

Sterility Testing + Validation （无菌检查验证）

The medium is tested with suitable strains of microrganisms inoculating <=100cfu and incubating at 20-25°C for not more than 3 days in case of bacteria and not more than 5 days in case of fungi.

培养基采用接种量≤100cfu的适当的微生物菌株进行检查。细菌在20-25°C孵育不超过3天，真菌不超过5天。

Cultural Response （培养反应）

Storage and Shelf Life （储存与保质期）

Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry date on label.

30°C以下密闭保存，制备好的培养基在2-8°C保存。标签上的截止日期前使用。

Reference （参考文献）

1.MacFaddin, J. F. 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria vol. 1. Baltimore: Williams and Wilkins.

2.The United States Pharmacopeia, 2008, USP31/NF26, The United States Pharmacopeial Convention, Rockville, MD. 3.Indian Pharmacopeia, 2007, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India.

4.Forbes, B. A., Sahm, D. F . and Weissfield, A. S. 2002. Bailey and Scott's Diagnostic Microbiology. 11 ed. St Louis: The C.V. Mosby Co.

Revision : 1 / 2011

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia? publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. HiMedia? Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

附表1：植物源性TSB参考报价

产品2、植物源性脑心浸出液肉汤 （植物源性BHI）

Brain Heart Infusion Broth, HiVeg/ Brain Heart              

货号：MV210/MV1036/MV1037

适合对营养要求不高的普通菌种以及对营养挑剔的脑膜炎奈瑟菌、肺炎链球菌、酿脓链球菌、金黄色葡萄球菌等菌种的生长繁殖。

Infusion, with 0.1% Agar, HiVeg^TM / with 6.5% NaCl, HiVeg^TM

Brain Heart Infusion Broth, HiVeg / Brain Heart Infusion, with 0.1% Agar, HiVeg / with 6.5% NaCl, HiVeg is employed for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations.

植物源性脑心浸出液肉汤 / 含0.1%琼脂的植物源性脑心浸出液肉汤/含6.5% NaCl的植物源性脑心浸出液肉汤主要用于繁殖对营养挑剔的病原球菌和其他与血培养相关的微生物及联合病理检查。

Final pH (at 25°C) 7.4 ± 0.2（最终pH值 (25°C) 7.4 ± 0.2）

** Formula adjusted, standardized to suit performance parameters

Directions :

Suspend 37.0 grams of MV210 or 38.0 grams of MV1036 or 97.0 grams of MV1037 in 1000 ml distilled water. Dispense into bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.

在1000ml蒸馏水中重悬37.0克MV210或38.0克MV1036或97.0克MV1037。用三角烧瓶或试管分装，并15磅压力（121℃）下高压灭菌15分钟。为了保证**效果，**配制当天就使用，否则需要蒸/煮几分钟，冷却后方可使用。

Principle and Interpretation (原理和说明)：

These media are prepared by completely replacing animal based peptone with vegetable peptone making the media free of BSE / TSE risks. Rosenow (1) devised the original medium by adding brain tissue to dextrose broth. These media like the conventional media are nutritious and well buffered to support the growth of wide variety of microorganisms (2, 3, 4). With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (5) and other fungi. In the formulation containing 6.5% sodium chloride (MV1037), the salt acts as a differential and/or selective agent by interfering with membrane permeability and osmotic and electrokinetic equilibria in salt intolerant organisms. The addition of 0.1% agar improves growth of microaerophilic and anaerobic microorganisms (4). Brain Heart Infusion Broth, HiVeg with addition of 1.5% agar should not be used for detection of haemolytic activity of Streptococci, since it contains dextrose, which has been reported to cause a typical haemolytic reactions when it is present in blood containing media. For selective isolation of fungi, addition of Gentamicin and/or Chloramphenicol is recommended (6).

这些培养基通过用植物蛋白胨完全替代动物源蛋白胨而制备，从而不具有疯牛病风险。 Rosenow通过将脑组织添加到葡萄糖肉汤中设计了最初的培养基。这些培养基和传统培养基一样富有营养和良好的缓冲能力，支持各种微生物的生长。加入10％去纤维绵羊血后，可用于荚膜组织胞浆菌等真菌的分离培养。在含有6.5％氯化钠（MV1037）的配方中，该盐干扰不耐盐微生物的膜渗透性和渗透压来作为差异性选择因子。添加0.1％的琼脂（MV1036）可改善微量需氧菌和厌氧菌的生长。添加1.5％琼脂的HiVeg脑心浸出液肉汤不应用于检测链球菌的溶血活性，因为它含有的葡萄糖在含血培养基中据报道会引起典型的溶血反应。对于选择性分离真菌，建议添加庆大霉素和/或氯霉素。

Quality Control :

Appearance of Powder

Yellow coloured may have slightly greenish tinge, homogeneous, free flowing powder.

Colour and Clarity

Light amber coloured, clear to slightly opalescent solution.

Reaction

Reaction of 3.7% w/v of MV210, 3.8%w/v of MV1036 or 9.7%w/v of MV1037 aqueous solution is pH 7.4 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.

Key : *= on MV210, MV1036

**= on MV1037

MV210Brain Heart Infusion Broth, HiVeg

1.Control

2.Neisseria meningitidis

3.Streptococcus pneumoniae

4.Streptococcus pyogenes

5.Staphylococcus aureuss

References:

1.Rosenow, 1919, J. Dental Research, 1:205.

2.Roseburg T. et al, 1944, J. Inf. Dis., 74:131.

3.Conant N.F., 1950, Diagnostic Procedures and Reagents, 3rd  ed., A.P.H.A. Inc.,New York.

4.MacFaddin  J.F.,  1985,  Media  for  Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.

5.Howard B., Keiser J.F., Weissfeld A., et al, 1994, Clinical and Pathogenic Microbiology, 2nd ed., Mosby Co.

6.Murray PR., Baron, Pfaller, Tenover and Yolken (Eds.), ASM, W^{ashington, D.C.}2003, In Manual of clinical Microbiology, 8th ed.

附表2：植物源性脑心浸出液肉汤（植物源性BHI）  参考报价

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