Effect of fetal calf serum on cytokine release by bone marrow-derived macrophages during infection w

骨髓来源的巨噬细胞（BMM）包括人口的静态细胞可以通过定义信号激活。在这里，我们直接比较趋化因子和单核因子的释放由BMM复活了在无血清培养基或在无血清培养基中单核细胞增生李斯特菌EGD或卡介苗感染。我们专注于这个问题，因为已经出现了由BMM由于不同的体外培养条件下，细胞因子的产生的一些有争议的报告。培养在无血清培养基中引物BMM为单核细胞趋化蛋白（MCP）释放1，白细胞介素（IL）- 6，和IL-12，但对巨噬细胞炎性蛋白没有影响（MIP）-1和肿瘤坏死因子（TNF）-针对单增李斯特菌感染α生产。与结核分枝杆菌感染后，BMM提高和刺激在无血清培养基中分泌更高水平的MCP-1、MIP-1α、IL-6和TNF-α，但不是IL-12与BMM在无血清培养基中培养和感染。血清的作用可能部分模仿干扰素γ。因为负责BMM启动的血清成分不明确，BMM培养在无血清的条件下提供确定巨噬细胞活化信号的适当的细胞。

英文原文：

Bone marrow-derived macrophages (BMM) comprise a population of quiescent cells which can be activated by defined signals. Here, we directly compare the release of chemokines and monokines by BMM raised either in serum-supplemented or in serum-free medium in response to Listeria monocytogenes EGD or Mycobacterium bovis BCG infection. We focused on this issue because there have been several controversial reports on the production of cytokines by BMM due to different in vitro culture conditions. Culture in serum-supplemented medium primed BMM for release of monocyte chemoattractant protein (MCP)-1, interleukin (IL)-6, and IL-12, but had no effect on macrophage inflammatory protein (MIP)-1alpha and tumor necrosis factor (TNF)-alpha production in response to L. monocytogenes infection. After challenge infection with M. bovis, BMM raised and stimulated in serum-supplemented medium secreted higher levels of MCP-1, MIP-1alpha, IL-6, and TNF-alpha but not of IL-12 as compared to BMM cultured and infected in a serum-free medium. The effects of serum could be partially mimicked by interferon-gamma. Because the serum components responsible for BMM priming are undefined, BMM cultured under serum-free conditions provide an appropriate cell population for defining macrophage activating signals.