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Treatment and control of mycoplasma contamination in Plasmodium falciparum culture2016-09-07 13:34
缔一生物导读:四抗生素的疗效比较,Plasmocin(大环内酯类),成分1,2,(四环素),和biomyc-3,支原体祛除剂(喹诺酮衍生物)是排除恶性疟原虫培养支原体检测。采用酶PCR支原体检测试剂盒和检测疟原虫生存存在支原体测定在姬姆萨染色涂片由处理的和未处理的文化。据观察,结合成分1和2杀死疟疾寄生虫的24小时内,而造成的死亡和biomyc-3 Plasmocin慢疟原虫长达6天。**的化合物,没有杀死疟疾寄生虫恶性疟原虫和根除支原体培养观察到MRA。 英文原文: A comparative efficacy of four antibiotics, plasmocin (macrolid), Biomyc-1, -2, (tetracycline), and Biomyc-3, and Mycoplasma Removing Agent (quinolone derivatives) was determined for elimination of mycoplasma from Plasmodium falciparum culture. Presence of mycoplasma was detected using enzyme-PCR-based mycoplasma detection kit and survival of malaria parasite was determined in Giemsa’s stained smear made from treated and untreated cultures. It was observed that a combination of Biomyc-1 and -2 killed malaria parasites within 24 h, whereas plasmocin and Biomyc-3 caused slow death of malaria parasite stretched over a period of 6 days. The only compound which did not kill malaria parasite and eradicated mycoplasma from P. falciparum culture was observed to be MRA. 推荐阅读:
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