丙型肝炎病毒在细胞培养中的完全复制

让缔一生物跟你们一起解密丙型肝炎病毒在细胞培养中的完全复制吧！

在丙型肝炎病毒（丙型肝炎病毒）生命周期的许多方面还没有被复制在细胞培养，这已经减缓了这一重要的人类病原体的研究进展。在这里，我们描述了一个全长HCV基因组复制并产生细胞培养中的病毒颗粒（病毒）。复制HCVcc是强大的，在48小时内生产近105感染单位每毫升。病毒颗粒的过滤、中和与病毒糖蛋白E2单克隆抗体。病毒进入依赖于一个假定的HCV受体细胞表达CD81。病毒的复制是由干扰素α的HCV特异性抗病毒化合物的抑制，这表明在体外系统将有助于提高抗病毒药物的搜索。（友情提示：了解细胞培养基，请点击这里）

英文原文：

Complete Replication of Hepatitis C Virus in Cell Culture

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 105 infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-α and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

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