胎牛血清胎球蛋白的钙结合性能

　　胎球蛋白，一个丰富的蛋白质胎牛血浆，是人类α2HS糖蛋白（α2HS）的牛同源。尽管许多研究，这两种蛋白质的生物学功能仍然无法实现。我们现在报告在胎牛血清胎球蛋白已印迹上电泳后的膜的显着的45Ca提高结合活性。纯化的蛋白质的钙结合能力通过平衡透析，这表明，牛胎球蛋白有多种钙结合位点，其中之一具有0.95×10-4M的Kd值分析据还表明，胎球蛋白的钙结合活性高于在晚期妊娠阶段白蛋白在牛胎儿血清的更大。由于胎牛血清不仅含有高浓度的胎球蛋白而且钙的高浓度，可能的是胎球蛋白功能通过钙离子浓度的正常化保持在胎儿血清高水平钙。

　　英文原文：

Calcium-binding properties of fetuin in fetal bovine serum        

Fetuin, an abundant protein in fetal bovine plasma, is the bovine homolog of human α2HS glycoprotein (α2HS). In spite of numerous studies, the biological functions of both proteins remain elusive. We now report the remarkable 45Ca-binding activity of fetuin in fetal bovine serum that has been blotted on a membrane after electrophoresis. The Ca-binding ability of the purified protein was analyzed by equilibrium dialysis, which revealed that bovine fetuin had multiple Ca-binding sites, one of which had a Kd of 0.95 × 10?4 M. It was also shown that the Ca-binding activity of fetuin was greater than that of albumin in serum of the bovine fetus at the late gestational stage. Since fetal bovine serum contains not only a high concentration of fetuin but also a high concentration of Ca, it is possible that fetuin functions to maintain high levels of Ca in fetal serum via normalization of the concentration of Ca2+ ions.

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