预防和清除支原体污染的细胞培养方法

Quarantine new cells of any origin

As described in previous sections, new cells which are brought from other laboratories are characterized as a main source of contamination. So, it is necessary to quarantine cells and check for mycoplasma contamination before using them for any purposes .

Reduce aerosol generation

Aerosols during manipulation of cells in the laminar hood or in the incubators and also aerosols made by personnel can transfer mycoplasma contamination in the lab. Therefore, avoiding activities which result in making aerosols can help to prevent mycoplasma contamination in cell cultures .

Importance of mycoplasma tests

Mycoplasma contamination rate in cells which are in use or are banked in cell banks in USA as well as in Europe is about 15 to 35%, according to reports .

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